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Delivering the Added Dimension in Drug Discovery Services

Surface Plasmon Resonance (SPR) Facilities
at Jubilant Biosys 

Biophysical Facility at Jubilant Biosys provides highly sensitive, label-free and real-time assessment of biomolecular interaction through the Surface Plasmon Resonance (SPR)  technology/platform. Our SPR services are carried out by our experienced scientific team that guides you through your project for various applications ranging from small molecule binding kinetics (reversible/irreversible), fragments screening, protein aggregation, drug competition assay etc . With having extensive knowledge in the drug discovery process, Jubilant Biosys offers tailored solutions to complex challenges that help in delivering successful results and achieve our mission.

SPR services

SPR Services

Proteins are important biological macromolecules as they play key roles in almost every biological process of a living organism. Determining and understanding the 3D structure of a protein is essential for deciphering the mechanistic details about how a protein performs its activities.

In small molecule drug discovery of any therapeutic area, it is a prerequisite to produce an enzymatically active recombinant protein that is equivalent to the native enzyme to understand its function/activity in an in-vitro setting.

Towards this, the Structural Biology team at Jubilant Biosys provides:

  • enzymatically active recombinant soluble proteins, overexpressed from E. coli or baculovirus-insect cells or mammalian cells.
  • co-crystal structures of any soluble recombinant protein through X-ray crystallography using in-house X-ray diffraction facility, as well as accessing Synchrotron beam at Australia and France.
  • stable cell lines that express protein-of-interest.

This team has the experience of working with more than 20 pharmaceutical/biotech companies worldwide and made the following success stories.

  • Solved 11 novel structures and 5 fab complex structures
  • Delivered > 300 proteins for assays from ~ 80 different targets
  • Delivered > 250 co-crystal structures for ~40 different targets
  • Generated ~400 clones
  • 7 publications in peer reviewed international journals

Currently, the Structural Biology team has been focusing on biophysical platform (SPR, Surface Plasmon Resonance; using Pioneer FE system) to facilitate FBDD (Fragment Based Drug Discovery) support to clients.

 

 

An optical phenomenon, SPR, enables binding activity monitoring of any two unlabelled molecules in real-time. The generated signal is dependent on changes in the refractive index at the surface of a gold sensor chip, as an analyte flows in a microfluidic channel and binds to a ligand immobilized on the sensor chip. Consequently, over time, plotting the binding response vs time called sensorgram. The sensorgram aids in the calculation of binding parameters, such as KD, kon and koff.

Advantages of SPR Technology

  • Deeper insight and understanding of molecular interactions – Real-time monitoring
  • Label-free detection
  • Small sample sizes
  • Reusable sensor chips
  • Replicate measurements consistently

Analysis for which the SPR services can be applied for

On top of the kinetic constants, SPR measurements can be applied for:

  • Determination of affinity
  • Assessment of irreversible compounds
  • High Throughput Screening of Fragments
  • Competition assay