DMPK

Drug Metabolism and Pharmacokinetics (DMPK) team has state-of-art facility with strong scientific team and infrastructure.

Drug Metabolism and Pharmacokinetics (DMPK) is an integral part of Drug discovery where its central role is to contribute to the optimization of compounds for man by balancing the properties associated with drug gastrointestinal absorption (for orally delivered therapies), distribution, clearance, elimination and DDI potential as rapidly and cost effectively as possible.

ADME DMPK Services

DMPK team supports both integrated preclinical discovery programs and fee-for-service projects and delivers quality and reproducible results with a quick turnaround time. DMPK team basically comprises in vitro, in vivo, met-ID and pre-formulation groups managed by highly experienced and matured scientific people. This team guides the discovery projects by trouble shooting the scientific issues and offer changes on scaffolds to drive the discovery projects in a right direction and de-risk the failures in later stage of development are not due to DMPK related issues.

Jubilant’s DMPK technical, research and development services meet the highest standards of professional performance to satisfy the unique requirements of our clients. We work closely with our clients to identify their requirements and clarify their expectations, including cost and time constraints. This DMPK screening services team has published several research articles in peer reviewed international journals and presented posters in conferences.

In Vitro ADME Services

The team, at Jubilant Biosys has state-of-art facility with strong scientific team and infrastructure offering In Vitro ADME services (Absorption, Distribution, Metabolism and Excretion). DMPK team supports both integrated preclinical discovery programs and stand-alone fee-for-service projects and deliver quality and reproducible results with a quick turnaround time.

This team has expertise to guide the discovery projects starting from hit identification, hit to lead optimization and then lead optimisation leading to identification of clinical candidates by trouble shooting the scientific issues and offer changes on scaffolds for a quick go/no-go decision at discovery stage and de-risk the failures at development stage.

In Vitro ADME Screening

The team has a track record to carry various in vitro studies or in vitro ADME screening at various stages of the drug discovery and development process to flag potential issues such as

  • Physiochemical studies - Kinetic solubility, thermodynamic solubility in PBS, SGF, SIF, chemical stability, early formulation assessment etc.
  • In vitro metabolism services - Metabolic stability using liver microsomes /hepatocytes/rCYPs/S-9 fraction, CYP inhibition, CYP phenotyping and CYP induction/PXR activation study to understand the involvement of CYP enzymes, blood/plasma/brain/tissue /microsomal protein binding, stability in plasma, buffer, blood, blood/plasma ratio, GSH adduct, TDI etc.
  • In vitro permeability and transporter studies - PAMPA, MDR 1-MDCK/ Caco-2 permeability, inhibition and substrate assessment of P-gp and BCRP transporters.
  • In vivo ADME screening services - PK studies by various routes, dose range finding studies, brain exposure study, BBB study etc.
Jubilant’s DMPK technical, research and development in vivo and in vitro drug metabolism services meet the highest standards of professional performance to satisfy the unique requirements of our clients.
 
We work closely with our clients to identify their requirements and fulfil their expectations, including cost and time constraints while maintaining highest quality standards. This team has published several research articles in peer reviewed international journals and presented posters in conferences.

ADME Surrogates

Physicochemical properties

  • Solubility (Thermodynamic and Kinetic method) 
    Various pH 
    Biorelevant medium (FaSSIF, FaSSGF, FeSSIF)
  • Log D (Octanol/water and Cyclohexane/water)
  • Chemical Stability
  • Blood Partitioning

In vitro Permeability

  • PAMPA
  • Caco-2
  • MDCK (wild type)

Animal Pharmacokinetics

  • Pharmacokinetics by various routes viz., oral, intravenous, intraperitoneal, subcutaneous in mice (Swiss Albino, C57, Balb/C), rats (Wistar, Sprague Dawley), rabbits (New Zealand white), dogs (Beagle) etc.
  • Tissue Distribution
  • Brain to Plasma Ratio
  • Biliary Excretion
  • Mass Balance

Protein Binding

  • Plasma Protein Binding (ultra Filtration and Equilibrium Dialysis)
  • Microsomal Protein Binding

In vitro Metabolism

  • Cytochrome P450 Inhibition
  • Cytochrome P450 Time Dependent Inhibition
  • Metabolic Stability 
    Liver Microsomes 
    S-9 Fraction
    Cryopreserved Hepatocyte
  • Plasma Stability
  • GSH Trapping

Metabolite ID

  • Identification In Vitro (Microsomes, S9 and Hepatocytes) & In Vivo Samples

 

Click here to download details about our capabilities in “Drug Metabolism and Pharmacokinetics (DMPK)”.

Click here to get in touch with us to obtain detailed proposal for your project